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u133 plus2 microarray  (Thermo Fisher)


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    Thermo Fisher u133 plus2 microarray
    Fam170a is located in the nucleus of spermatids at steps 9–13 and has a low level of mRNA in human sperm from individuals with teratozoospermia. ( A , B ) Immunofluorescence staining displayed the localization and distribution of Fam170a protein in mouse and human seminiferous tubules, respectively. Roman numerals indicate the spermatogenic stages of the tubules in WT testicular sections. S: spermatid. The specific stages of mouse tubules were determined according to published criteria [ , ]. ( C ) Schematic summary of Fam170a protein localization during spermiogenesis. ( D ) Three different microarrays captured Fam170a gene transcription levels in sperm samples from normal and teratozoospermia men. The original data source is marked at the top of the histogram. GDS2695 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2695±FAM170A ), GDS2696 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2696+FAM170A ), and <t>GDS2697</t> ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2697+FAM170A ); ** P < .01, * P < .005. The data are displayed as mean ± SEM. A t -test was performed, and false discovery rate (FDR) P -values were shown. ( E ) The relative Fam170a mRNA abundance in sperm samples from normal ( n = 4) and teratozoospermia ( n = 3) men after qRT-PCR analysis. The control data are displayed as mean ± SEM.
    U133 Plus2 Microarray, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/u133 plus2 microarray/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    u133 plus2 microarray - by Bioz Stars, 2026-04
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    Images

    1) Product Images from "Fam170a deficiency causes male infertility by impairing histone-to-protamine exchange during mouse spermiogenesis"

    Article Title: Fam170a deficiency causes male infertility by impairing histone-to-protamine exchange during mouse spermiogenesis

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkaf023

    Fam170a is located in the nucleus of spermatids at steps 9–13 and has a low level of mRNA in human sperm from individuals with teratozoospermia. ( A , B ) Immunofluorescence staining displayed the localization and distribution of Fam170a protein in mouse and human seminiferous tubules, respectively. Roman numerals indicate the spermatogenic stages of the tubules in WT testicular sections. S: spermatid. The specific stages of mouse tubules were determined according to published criteria [ , ]. ( C ) Schematic summary of Fam170a protein localization during spermiogenesis. ( D ) Three different microarrays captured Fam170a gene transcription levels in sperm samples from normal and teratozoospermia men. The original data source is marked at the top of the histogram. GDS2695 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2695±FAM170A ), GDS2696 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2696+FAM170A ), and GDS2697 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2697+FAM170A ); ** P < .01, * P < .005. The data are displayed as mean ± SEM. A t -test was performed, and false discovery rate (FDR) P -values were shown. ( E ) The relative Fam170a mRNA abundance in sperm samples from normal ( n = 4) and teratozoospermia ( n = 3) men after qRT-PCR analysis. The control data are displayed as mean ± SEM.
    Figure Legend Snippet: Fam170a is located in the nucleus of spermatids at steps 9–13 and has a low level of mRNA in human sperm from individuals with teratozoospermia. ( A , B ) Immunofluorescence staining displayed the localization and distribution of Fam170a protein in mouse and human seminiferous tubules, respectively. Roman numerals indicate the spermatogenic stages of the tubules in WT testicular sections. S: spermatid. The specific stages of mouse tubules were determined according to published criteria [ , ]. ( C ) Schematic summary of Fam170a protein localization during spermiogenesis. ( D ) Three different microarrays captured Fam170a gene transcription levels in sperm samples from normal and teratozoospermia men. The original data source is marked at the top of the histogram. GDS2695 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2695±FAM170A ), GDS2696 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2696+FAM170A ), and GDS2697 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2697+FAM170A ); ** P < .01, * P < .005. The data are displayed as mean ± SEM. A t -test was performed, and false discovery rate (FDR) P -values were shown. ( E ) The relative Fam170a mRNA abundance in sperm samples from normal ( n = 4) and teratozoospermia ( n = 3) men after qRT-PCR analysis. The control data are displayed as mean ± SEM.

    Techniques Used: Immunofluorescence, Staining, Quantitative RT-PCR, Control



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    Fam170a is located in the nucleus of spermatids at steps 9–13 and has a low level of mRNA in human sperm from individuals with teratozoospermia. ( A , B ) Immunofluorescence staining displayed the localization and distribution of Fam170a protein in mouse and human seminiferous tubules, respectively. Roman numerals indicate the spermatogenic stages of the tubules in WT testicular sections. S: spermatid. The specific stages of mouse tubules were determined according to published criteria [ , ]. ( C ) Schematic summary of Fam170a protein localization during spermiogenesis. ( D ) Three different microarrays captured Fam170a gene transcription levels in sperm samples from normal and teratozoospermia men. The original data source is marked at the top of the histogram. GDS2695 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2695±FAM170A ), GDS2696 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2696+FAM170A ), and <t>GDS2697</t> ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2697+FAM170A ); ** P < .01, * P < .005. The data are displayed as mean ± SEM. A t -test was performed, and false discovery rate (FDR) P -values were shown. ( E ) The relative Fam170a mRNA abundance in sperm samples from normal ( n = 4) and teratozoospermia ( n = 3) men after qRT-PCR analysis. The control data are displayed as mean ± SEM.
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    Fam170a is located in the nucleus of spermatids at steps 9–13 and has a low level of mRNA in human sperm from individuals with teratozoospermia. ( A , B ) Immunofluorescence staining displayed the localization and distribution of Fam170a protein in mouse and human seminiferous tubules, respectively. Roman numerals indicate the spermatogenic stages of the tubules in WT testicular sections. S: spermatid. The specific stages of mouse tubules were determined according to published criteria [ , ]. ( C ) Schematic summary of Fam170a protein localization during spermiogenesis. ( D ) Three different microarrays captured Fam170a gene transcription levels in sperm samples from normal and teratozoospermia men. The original data source is marked at the top of the histogram. GDS2695 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2695±FAM170A ), GDS2696 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2696+FAM170A ), and <t>GDS2697</t> ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2697+FAM170A ); ** P < .01, * P < .005. The data are displayed as mean ± SEM. A t -test was performed, and false discovery rate (FDR) P -values were shown. ( E ) The relative Fam170a mRNA abundance in sperm samples from normal ( n = 4) and teratozoospermia ( n = 3) men after qRT-PCR analysis. The control data are displayed as mean ± SEM.
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    Fam170a is located in the nucleus of spermatids at steps 9–13 and has a low level of mRNA in human sperm from individuals with teratozoospermia. ( A , B ) Immunofluorescence staining displayed the localization and distribution of Fam170a protein in mouse and human seminiferous tubules, respectively. Roman numerals indicate the spermatogenic stages of the tubules in WT testicular sections. S: spermatid. The specific stages of mouse tubules were determined according to published criteria [ , ]. ( C ) Schematic summary of Fam170a protein localization during spermiogenesis. ( D ) Three different microarrays captured Fam170a gene transcription levels in sperm samples from normal and teratozoospermia men. The original data source is marked at the top of the histogram. GDS2695 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2695±FAM170A ), GDS2696 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2696+FAM170A ), and <t>GDS2697</t> ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2697+FAM170A ); ** P < .01, * P < .005. The data are displayed as mean ± SEM. A t -test was performed, and false discovery rate (FDR) P -values were shown. ( E ) The relative Fam170a mRNA abundance in sperm samples from normal ( n = 4) and teratozoospermia ( n = 3) men after qRT-PCR analysis. The control data are displayed as mean ± SEM.
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    Fam170a is located in the nucleus of spermatids at steps 9–13 and has a low level of mRNA in human sperm from individuals with teratozoospermia. ( A , B ) Immunofluorescence staining displayed the localization and distribution of Fam170a protein in mouse and human seminiferous tubules, respectively. Roman numerals indicate the spermatogenic stages of the tubules in WT testicular sections. S: spermatid. The specific stages of mouse tubules were determined according to published criteria [ , ]. ( C ) Schematic summary of Fam170a protein localization during spermiogenesis. ( D ) Three different microarrays captured Fam170a gene transcription levels in sperm samples from normal and teratozoospermia men. The original data source is marked at the top of the histogram. GDS2695 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2695±FAM170A ), GDS2696 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2696+FAM170A ), and <t>GDS2697</t> ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2697+FAM170A ); ** P < .01, * P < .005. The data are displayed as mean ± SEM. A t -test was performed, and false discovery rate (FDR) P -values were shown. ( E ) The relative Fam170a mRNA abundance in sperm samples from normal ( n = 4) and teratozoospermia ( n = 3) men after qRT-PCR analysis. The control data are displayed as mean ± SEM.
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    Fam170a is located in the nucleus of spermatids at steps 9–13 and has a low level of mRNA in human sperm from individuals with teratozoospermia. ( A , B ) Immunofluorescence staining displayed the localization and distribution of Fam170a protein in mouse and human seminiferous tubules, respectively. Roman numerals indicate the spermatogenic stages of the tubules in WT testicular sections. S: spermatid. The specific stages of mouse tubules were determined according to published criteria [ , ]. ( C ) Schematic summary of Fam170a protein localization during spermiogenesis. ( D ) Three different microarrays captured Fam170a gene transcription levels in sperm samples from normal and teratozoospermia men. The original data source is marked at the top of the histogram. GDS2695 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2695±FAM170A ), GDS2696 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2696+FAM170A ), and <t>GDS2697</t> ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2697+FAM170A ); ** P < .01, * P < .005. The data are displayed as mean ± SEM. A t -test was performed, and false discovery rate (FDR) P -values were shown. ( E ) The relative Fam170a mRNA abundance in sperm samples from normal ( n = 4) and teratozoospermia ( n = 3) men after qRT-PCR analysis. The control data are displayed as mean ± SEM.
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    Fam170a is located in the nucleus of spermatids at steps 9–13 and has a low level of mRNA in human sperm from individuals with teratozoospermia. ( A , B ) Immunofluorescence staining displayed the localization and distribution of Fam170a protein in mouse and human seminiferous tubules, respectively. Roman numerals indicate the spermatogenic stages of the tubules in WT testicular sections. S: spermatid. The specific stages of mouse tubules were determined according to published criteria [ , ]. ( C ) Schematic summary of Fam170a protein localization during spermiogenesis. ( D ) Three different microarrays captured Fam170a gene transcription levels in sperm samples from normal and teratozoospermia men. The original data source is marked at the top of the histogram. GDS2695 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2695±FAM170A ), GDS2696 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2696+FAM170A ), and <t>GDS2697</t> ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2697+FAM170A ); ** P < .01, * P < .005. The data are displayed as mean ± SEM. A t -test was performed, and false discovery rate (FDR) P -values were shown. ( E ) The relative Fam170a mRNA abundance in sperm samples from normal ( n = 4) and teratozoospermia ( n = 3) men after qRT-PCR analysis. The control data are displayed as mean ± SEM.
    U133 Plus2 Microarrays, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fam170a is located in the nucleus of spermatids at steps 9–13 and has a low level of mRNA in human sperm from individuals with teratozoospermia. ( A , B ) Immunofluorescence staining displayed the localization and distribution of Fam170a protein in mouse and human seminiferous tubules, respectively. Roman numerals indicate the spermatogenic stages of the tubules in WT testicular sections. S: spermatid. The specific stages of mouse tubules were determined according to published criteria [ , ]. ( C ) Schematic summary of Fam170a protein localization during spermiogenesis. ( D ) Three different microarrays captured Fam170a gene transcription levels in sperm samples from normal and teratozoospermia men. The original data source is marked at the top of the histogram. GDS2695 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2695±FAM170A ), GDS2696 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2696+FAM170A ), and <t>GDS2697</t> ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2697+FAM170A ); ** P < .01, * P < .005. The data are displayed as mean ± SEM. A t -test was performed, and false discovery rate (FDR) P -values were shown. ( E ) The relative Fam170a mRNA abundance in sperm samples from normal ( n = 4) and teratozoospermia ( n = 3) men after qRT-PCR analysis. The control data are displayed as mean ± SEM.
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    Image Search Results


    Fam170a is located in the nucleus of spermatids at steps 9–13 and has a low level of mRNA in human sperm from individuals with teratozoospermia. ( A , B ) Immunofluorescence staining displayed the localization and distribution of Fam170a protein in mouse and human seminiferous tubules, respectively. Roman numerals indicate the spermatogenic stages of the tubules in WT testicular sections. S: spermatid. The specific stages of mouse tubules were determined according to published criteria [ , ]. ( C ) Schematic summary of Fam170a protein localization during spermiogenesis. ( D ) Three different microarrays captured Fam170a gene transcription levels in sperm samples from normal and teratozoospermia men. The original data source is marked at the top of the histogram. GDS2695 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2695±FAM170A ), GDS2696 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2696+FAM170A ), and GDS2697 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2697+FAM170A ); ** P < .01, * P < .005. The data are displayed as mean ± SEM. A t -test was performed, and false discovery rate (FDR) P -values were shown. ( E ) The relative Fam170a mRNA abundance in sperm samples from normal ( n = 4) and teratozoospermia ( n = 3) men after qRT-PCR analysis. The control data are displayed as mean ± SEM.

    Journal: Nucleic Acids Research

    Article Title: Fam170a deficiency causes male infertility by impairing histone-to-protamine exchange during mouse spermiogenesis

    doi: 10.1093/nar/gkaf023

    Figure Lengend Snippet: Fam170a is located in the nucleus of spermatids at steps 9–13 and has a low level of mRNA in human sperm from individuals with teratozoospermia. ( A , B ) Immunofluorescence staining displayed the localization and distribution of Fam170a protein in mouse and human seminiferous tubules, respectively. Roman numerals indicate the spermatogenic stages of the tubules in WT testicular sections. S: spermatid. The specific stages of mouse tubules were determined according to published criteria [ , ]. ( C ) Schematic summary of Fam170a protein localization during spermiogenesis. ( D ) Three different microarrays captured Fam170a gene transcription levels in sperm samples from normal and teratozoospermia men. The original data source is marked at the top of the histogram. GDS2695 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2695±FAM170A ), GDS2696 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2696+FAM170A ), and GDS2697 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2697+FAM170A ); ** P < .01, * P < .005. The data are displayed as mean ± SEM. A t -test was performed, and false discovery rate (FDR) P -values were shown. ( E ) The relative Fam170a mRNA abundance in sperm samples from normal ( n = 4) and teratozoospermia ( n = 3) men after qRT-PCR analysis. The control data are displayed as mean ± SEM.

    Article Snippet: Similarly, the data from the Illumina Sentrix Expression (HumanRef-8) microbead microarray (five normal and eight teratozoospermia samples, GDS2696) and Affymetrix U133 plus2 microarray (13 normal and 8 teratozoospermia samples, GDS2697) revealed significantly decreased Fam170a mRNA abundance in the teratozoospermia samples (Fig. ; P < .05, P < .01).

    Techniques: Immunofluorescence, Staining, Quantitative RT-PCR, Control